Anti-Inflammatory evaluation of black rice extract inhibits TNF-α, IFN-γ and IL-6 cytokines produced by immunocompetent cells

Kurnia Hartati, Fadjar and Bambang Widjanarko, Simon and Widyaningsih, Tri Dewanti and Rifa’i, Muhaimin (2017) Anti-Inflammatory evaluation of black rice extract inhibits TNF-α, IFN-γ and IL-6 cytokines produced by immunocompetent cells. Food and Agricultural Immunology (12 Jun). ISSN 0954-0105

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Abstract

Black rice has been known for its many beneficial effects to our body because it contains several chemical compounds that act as anti- inflammatory agents. This study aims to compare the anti- inflammatory activity of ethanol extract and aqueous extract of black rice. Anti-inflammatory activity test was performed in vitro on splenocytes of diabetes mellitus mice model as the control of inflammation. Cells were cultured in Roswell Park Memorial Institute medium with 10% fetal bovine serum, anti-CD3 and lipopolysaccharide and black rice extract 50, 100 and 200 μg/mL. In day 3, cells were harvested, stained and analyzed by flow cytometry. The result shows that the ethanol extract and aqueous extract of black rice have anti-inflammatory activity. 50 μg/mL black rice aqueous extract has the most extensive anti- inflammatory activity indicated by increased T reg cells, decreased nuclear factor kappa B activity on CD4 + , CD8 + T cells, decreased production of TNF-α by CD4 + T cells, decreased production of IL-6 and IFN-γ by macrophages.

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